Note: It is dangerous because it is an experiment to treat bacteria. Please experiment in the laboratory and other places under the supervision of experts.
- １．Srerilize a petri dish
- The dish was sterilized at 121℃. for 1 hour (using an autoclave).
(The machine on the front is an autoclave)
- ２．Make an agar culture area
- 3.5 g of the medium was added to 100 ml of purified water and dissolved
by warming. After dispensing into a flask, sterilized at 121℃. for 15 minutes,
it was poured into a petri dish.
- Pour in as short a time as possible to prevent bacteria from entering while entering a petri dish.
- The petri dish is written with a magic pen so that you can see where and where the environment is located.
- Pour in as short a time as possible to prevent bacteria from entering while entering a petri dish.
- ３．Collect bacteria
- Samples were collected using a tape with a lower adhesive strength. This time, I used hands washed with hands · soap before hand washing.
- ４．Put the time in the warmer
- [Room temperature (approximately 27 degrees)] [in a refrigerator (about 5 °)] and divided into each environment of the [while applying ultraviolet] [dark] for a period of time (2 days) and allowed to stand.
- ５．Observation
- You can not open the lid of a petri dish and check the condition from below.
Note: Opening the lid of a petri dish will contain other bacteria. - ６．Treatment
- Wear a mask · vinyl glove, treat tweezers and other agar culture areas
where fungi propagated while ventilating. The agar culture area was placed
in a plastic bag and sealed.
On the next page we will look at "Result of experiment [2]".
