Note: It is dangerous because it is an experiment to treat bacteria. Please experiment in the laboratory and other places under the supervision of experts.
- １．Sterilize a oetri dish
- The dish was sterilized at 121 ° C. for 1 hour (using an autoclave).
(The machine on the front is an autoclave)
- ２．Make an agar culture area
- 3.5 g of the medium was added to 100 ml of purified water and dissolved
by warming. After dispensing into a flask, sterilized at 121 ° C. for 15
minutes, it was poured into a petri dish.
- Pour in as short a time as possible to prevent bacteria from entering while entering a petri dish.
- I am filling in a petri dish with a magic pen so that you can see what kind of place it is.
- Pour in as short a time as possible to prevent bacteria from entering while entering a petri dish.
- ３．Collect bacteria
- Samples were taken using a tape with a lower adhesive strength.
- ４．Take time while keeping warm
- The temperature was kept for 1 day in a warmer kept at 37 degrees.
When placing in the warmer, place the lid of the petri dishes down.
- ５．Observation
- You can not open the lid of a petri dish and check the condition from below.
Note: Opening the lid of a petri dish will contain other bacteria. - ６．Treatment
- Wear mask / vinyl gloves, treat tweezers and other agar cultures where
bacteria propagated while ventilating. The agar culture area was placed
in a plastic bag and sealed.
On the next page we will look at "results of the experiment [1]".
